Year: 2021 | Month: February | Volume 11 | Issue 1
Phylogenetic Analysis of Bacillus anthracis Strains Isolated from Clinical and Environmental Samples in Andhra Pradesh
B. Gireesha
Devalam Rani Prameela
Bollini Sreedevi
DOI:10.30954/2277-940X.01.2021.14
Abstract:
Anthrax, caused by Bacillus anthracis, is a severe zoonosis with a great impact on animal and human health. In the present study, 15 out of 16 isolates from clinical ear piece and soil samples (16), 8,6 and one samples isolated respectively from bovine, sheep and goat were confirmed as Bacillus on amplification of rpoB gene, 22 isolates (ear piece13, soil-9) were PCR positive for PA gene of PXO and 10 isolates (ear piece) amplified CAP gene confirming Bacillus anthracis. Similarly, all 31 isolates (earpiece-15 and soil-16) were confirmed as Bacillus anthracis on amplifying rpoB gene and chromosomal Ba813 gene. On nucleotide analysis with genus specific rpoB gene of earpiece and soil isolates shown 99-100% identity, whereas nucleotide analysis with species specific genes; PA of PXO1, CAP of PXO2, rpoB and chromosomal Ba813 gene shown 98-100% identity with their respective reference strains of Genbank. On phylogenetic analysis, earpiece isolates and soil isolates from endemic districts of Andhra Pradesh shown close evolutionary relationship with each other. However, earpiece isolates of Sr6 (Accession No:MK310254) and N3 shared ancestral relation with global reference strains of USA (Accession No:CP012730) and Japan (Accession no: AP014833) respectively. Similarly soil isolate of VM9 also shared ancestral relation with global reference strain of Japan (Accession no: AP014833). This phylogenetic analysis deciphered that there is no strain variation among isolates of B. anthracis collected from different clinical and soil samples of different districts of Andhra Pradesh.
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